Substrate Concentration and Thermal Effects During Polyhydroxyalkanoate Bioproduction
DOI:
https://doi.org/10.53704/fujnas.v11i1.415Keywords:
Polymers, Polyhydroxyalkanoates (PHA), Bio-polyesters, Micrococcus flavus SS21B, OptimizationAbstract
Polyhydroxyalkanoates (PHAs) have long been thought to have the potential to replace petrochemical-based polymers because their production cost has been a challenging factor hindering their production processes. Previously characterized quantities of Micrococcus flavus SS21B screened for polyhydroxyalkanoates (PHAs) were utilized. PHAs were then synthesized using corn cobs as a carbon source. Proximate analysis of corn cobs undertaken during this research reveals the presence of moisture and ash contents, crude fibre and protein, and carbohydrates. Optimization studies reveal an optimal PHA production at 8% substrate concentration and 37°C. This study shows that the carbon source has a more significant effect on the production of PHAs; i.e., the higher the carbon source in the production, the higher the PHAs synthesized, and the thermal effect required is average and not in excess.
References
Arthy, S., Manoj L., Jin Y., Azlinah M., Doan V. & Kumar S. (2020). Can Polyhydroxyalkanoates Be Produced Efficiently from Waste Plant and Animal Oils, Frontier in Bioengineering and Biotechnology. 8, 169. Doi: 10.3389/fbioe.2020.00169
Akinwunmi, O., & Omotayo, F. (2016). Proximate Analysis and Nutritive Value of Ten Common Vegetables in South-West (Yoruba Land) Nigeria. Communications in Applied Sciences. ISSN 2201-7372. 4 (2), 79-91.
Ooi, D. J., Iqbal, S., & Ismail, M. (2012). Proximate composition, nutritional attributes and mineral composition of Peperomia pellucida L.(Ketumpangan Air) grown in Malaysia. Molecules, 17(9), 11139-11145.
Francisco R., Yuby C., Idoia E. & Juan F. Saldarriaga (2019). Kinetic Study of Corn and Sugarcane Waste Oxidative Pyrolysis. Energies, 12(23), 4594, https://doi.org/10.3390/en12234594
Jimoh, S. O., Akinwande, F. O., Ayinde O. A. & Senbadejo, T. Y. (2018). Polyhydroxyalkanoate Producing Potential of Saccharomyces cerevisiae. Fountain Journal of Natural and Applied Sciences 7(1), 42-47.
Joyline M., & Aruna K., (2019). Production and Characterization of Polyhydroxyalkanoates Bacillus Megaterium Strain Jha using Inexpensive Agro-industrial Wastes. International Journal of Recent Scientific Research, 10 (07A), pp.33359-33374, http:/dx.doi.ogr/10.24327/ijrsr.2019.1007.3656s
Lu-Ji, Y., Tao C. & Yanhong X. (2019). Effect of Corn Cobs as External Carbon Sources on Nitrogen Removal in Constructed Wetlands Treating Micro-Polluted River Water. Water Science and Technology, 79 (9), 1639–1647.
Muhammad, S, Panda, A. N. & Ray, L (2019). An investigation for recovery of (PHA) from Bacillus sp. BPPI-14 isolated from plastic waste landfill. International Journal of Biological Macromolecules 134(8). DOI: 10.1016/j.ijbiomac.2019.05.155.
Nwinyi, O., & Owolabi T., (2019). Scanning Electron Microscopy and Fourier Transmission Analysis of Polyhydroxyalkanoates Isolated from Bacteria Species from Abattoir in Ota, Nigeria. Journal of King Saud University-Science, 31 (3), 285-298.
Ojumu, T.V., Yu, J. & Solomon, B.O. (2004). Production of Polyhydroxyalkanoates, a bacterial biodegradable polymer African Journal of Biotechnology 3 (1), 18-24.
Otunola G., & Afolayan A. (2019). Proximate and Elemental Composition of Leaf, Corm, Root and Hypoxis hemerocallidea: A Southern Africa Multipurpose Medicinal Plant. Pakistan. Journal of Pharmaceutical. Science, 32 (2).
Rodrigues P. R. & Druzian J. I. (2018). Impact of Different Bacterial Strains on the Production, Composition, and Properties of Novel Polyhydroxyalkanoates Using Crude Palm Oil as Substrate. Chemical and Biochemical Engineering Quarterly, 32 (1).
Shreya, S. & Anil, K. (2021). Production and Characterization of Polyhydroxyalkanoates from Industrial Waste Using Soil Bacterial Isolates Brazilian Journal of Microbiology. 52 (2), 715–726. doi: 10.1007/s42770-021-00452-z PMCID: PMC8105478. PMID: 33590449
Songyuan Y., Suhang L. & Xiaoqiang J., (2019). Production of Medium Chain Length Polyhydroxyalkanoate from Acetate by Engineered Pseudomonas putida KT2440. Journal of Industrial Microbiology and Biotechnology, 46 (6), 793–800, https://doi.org/10.1007/s10295-019-02159-5.
Sneha P., Changam S. & Balakumuran M. (2017). Optimization, Purification and Characterization of Polyhydroxybutyrate (PHB) Produced by Bacillus Cereus Isolated from Sewage. International Journal of Chem Tech Research. 10(7), 884-904.
Su Y., Jiun Y., and Kumar S., (2017). Degradation of Polyhydroxyalkanoate (PHA). Journal of Siberian Federal University. Biology. 10(2), 211-225.
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