Antimicrobial and Phytochemical Analyses of Sida acuta Leaf Extracts on Selected Wound Isolates

F. A. Jimoh; W. T. Aborisade; A. A. Bamidele

doi:10.53704/fujnas.v10i1.347

Authors

F. A. Jimoh Department of Biosciences and Biotechnology (Microbiology Unit), Faculty of Pure and Applied Sciences, Kwara State University, Malete, Kwara State.
W. T. Aborisade Department of Biosciences and Biotechnology (Microbiology Unit), Faculty of Pure and Applied Sciences, Kwara State University, Malete, Kwara State
A. A. Bamidele Department of Biosciences and Biotechnology (Microbiology Unit), Faculty of Pure and Applied Sciences, Kwara State University, Malete, Kwara State

DOI:

https://doi.org/10.53704/fujnas.v10i1.347

Abstract

Medicinal plants have been intensively screened for their bioactivity in order to treat various diseases in human. This study was performed to evaluate the antimicrobial and phytochemical properties of Sida acuta leaf extracts against selected wound microbes. The plant extracts were obtained sequentially using n-hexane, aqueous and acetone respectively in order of increasing polarity. The extracts were tested for their antimicrobial activities against Staphylococcus aureus, Candida albicans, Klebsiella pneumoniae, Enterococcus faecium, Acinetobacter baumannii, Salmonella typhi and Pseudomonas aeruginosa using agar-well diffusion method. The sensitivity of the test organisms to the extracts was represented by zones of inhibitions (mm) at different concentrations. There was corresponding increase in the zones of inhibitions (mm) on the test organisms as the concentration of the extracts increased from 64 mg/mL – 512 mg/mL. Among the plant extracts, the aqueous extract of Sida acuta leaf revealed significantly higher zones of inhibitions (mm) from 7.50±2.12 - 25.00±1.40 on all isolates. This was closely followed by the acetone extract while the n-hexane extract produced the lowest zones of inhibitions (mm) on all the test organisms except for Acinetobacter baumannii which has 9.80±0.42 at the concentration of 512 mg/mL. Results obtained on the phytochemical analyses of the aqueous and acetone extracts revealed the presence of tannins, saponins, alkaloids and flavonoids. Hence, the antimicrobial effects of Sida acuta leaf extracts has been revealed in this study, therefore its controlled use should be encouraged in the treatment of wounds and other infections caused by these microorganisms.

Keywords: Antimicrobial properties, Isolates, Phytochemical analyses, Sida acuta

References

Ahmed, R.N., Jumah, S.S., Arekemase, M.O., Agbabiaka, T.O., Adam, A.I. & Adejoro, D.O. (2017). Serial Exhaustive Extraction: Influence of Solvent Polarity on Antibacterial Activity of Extracts of Leaves of Tithonia diversifolia Nigerian Journal of Pure and Applied Science. 30(1), 2987- 2995. doi: http://dx.doi.org/10.19240/njpas.2017.A05

Akinnibosun, F. I. & Pela, B. (2015). Evaluation of the phytochemicals and the antibacterial properties of Sida acuta leaf extracts and their effects on wound bacterial isolates. Chemsearch Journal, 6(2), 70-76.

Anyanwu, M. U. & Okoye, R. C. (2017). Antimicrobial activities of Nigerian medicinal plants. Journal of Intercult Ethnopharmarcol. 6(2), 240- 259.

Chinelo, A.E. & Miracle, O.E. (2018). Phytochemical and antimicrobial investigations on various parts of Sida acuta Burm. F. Journal of Ayurvedic and Herbal medicine, 4(2):71-75.

Ghaddar, N., Anastasiadis, E., Halimeh, R., Ghaddar, A., Dhar, R., AlFouzan, W., Yusef, H. & El Chaar, M., (2020). Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon. BMC Infectious Diseases, 20(1), 1-9.

Jayaseelan, E.C., Tharmila, S., Sathiyaseelan, V., & Niranjan, K.(2012). Antibacterial Activity of Various Solvent Extracts of some Selected Medicinal Plants present in Jaffna Peninsula. International Journal of Pharmaceutical Archives, 3(4), 792-796.

Jindal, A., Kumar, P. & Jain, C. (2012). Antifungal Activity of Flavonoids of Sida acuta Burm f. Against Candida albicans. International Journal of Drug Development and Research, 4(3), 92-96.

Kolapo, A.L., Okunade, M.B., Adejumobi J.A. & Ogundiya, M.O. (2009). Phytochemical Composition and Antimicrobial Activity of Prosopis Africana Against Some Selected Oral Pathogens. World Journal of Agricultural Sciences 5 (1), 90-93.

Mbajiuka, C. S., Obeagu, E. I., Ochei, K.C. & Nnadi, D.C. (2014). The antibacterial activity of leaf extracts of Ocimum gratissimum and S. acuta. International Journal of Microbiological research 5(2), 124-129.

Pooja, C., Kuppast, I.J., Virupaksha, J.H. & Ravi, M.C. (2015). A review on Sida acuta. International Journal of Universal Pharmacy and Biosciences 4(1), 36-48.

Raimi, M .M., Oyekanmi, A.M. & Adegoke, B.M. (2014). Proximate, Phytochemical and Micronutrient Composition of Sida Acuta. IOSR Journal of Applied Chemistry 7(2), 93-98.

Sarita, M., Shisir, L., & Raj, K.D. (2019). In-Vitro Antimicrobial Activity of some Medicinal Plants Against Human Pathogenic Bacteria. Journal of Tropical Medicine, 2(19),1-5.

Senthilkumar, R.P., Bhuvanashwari, V., Malayaman, V., Ranjithkumar, R. & Sathiyavimal, S. (2018). Phytochemical screening of aqueous leaf extract of Sida acuta Burm. F. and its antibacterial activity. JETIR Journal, 8(5), 2349-5162.

Shittu, & Alagbe, J.O. (2020). Phytonutritional profiles of Broom weed (Sida acuta) leaf extract. Annal of Clinical Toxicology, 3(2), 10-32.

Zakariyah, R.F., Sani, A., Odebisi-Omokanye, M.B. , Akolade, J.O. , Ahmed, R.N. Jimoh, F.A. & Badmus, S.A. (2017). Screening of Prosopis africana for antimicrobial activity against selected oralpathogens. Nigerian Journal of Pure and Applied Science 30(2), 3049-3058.