Antimicrobial Activities of Bryophyllum pinnatum on Some Selected Clinical Isolates.

I.  A. Ibikunle; K. S. Bolanle; A. A. Jumai; D. G. Ifeoluwa; I. I. Anibijuwon; B. K. Saliu; J. A. Abioye; I. D. Gbala

doi:10.53704/fujnas.v6i1.135

Authors

I. A. Ibikunle Department of Microbiology, Faculty of Life Sciences, University of Ilorin, P.M.B. 1515, Ilorin, Kwara State, Nigeria
K. S. Bolanle University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.
A. A. Jumai University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.
D. G. Ifeoluwa University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.
I. I. Anibijuwon University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.
B. K. Saliu University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.
J. A. Abioye University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.
I. D. Gbala University of Ilorin, Faculty of Life Sciences, Department of Microbiology, P.M.B. 1515, Ilorin, Kwara State, Nigeria.

DOI:

https://doi.org/10.53704/fujnas.v6i1.135

Abstract

Ethanol, methanol and aqueous extracts of the leaves of Bryophyllum pinnatum obtained through cold maceration, were screened for their antibacterial activities against selected multi-drug resistant bacteria (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi Staphylococcus aureus) using the agar well diffusion method. Broth dilution method was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts at concentrations ranging from 100mg/ml to 25mg/ml. The ethanol extract was the most reactive while the aqueous extract showed lesser antibacterial activity. Ofloxacin was the most effective antibiotic in the antibiotic susceptibility profiling of the test organisms. It was however evident that the ethanol extract of Bryophyllum pinnatum has higher antibacterial efficacy on the test organisms than Ofloxacin. Bacteriostatic and -cidal activities were exhibited by the plant extracts against the organisms ranging from 25 – 100 × 103 μg/ml. Succinctly, Bryophyllum pinnatum possesses biologically active constituents with explorable pharmacological potentials.

Keywords: Antimicrobial, Bryophyllum pinnatum, Extraction solvents, minimum inhibitory concentration (MIC), minimum bactericidal concentration

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